Colorimeter sensor

A simple, effective, recording colorimeter capable of monitoring changes in transmission or absorbance over time. 

Ideal for teaching the characteristics of enzyme kinetics and chemical rates of reaction. 

 
2-minute on Colorimeter - rate of reaction

Uses a white LED, standard cuvettes and coloured slides (4 supplied) as filters. Use the meters function of Easysense it becomes a standard reading display colorimeter.

Downloads Colorimeter Sensor Manual  Doc No.: DS042 | Issue: 7

Contents/Details:

• Colour filters are supplied as 35 mm slides, simple to add more filter colours.
• Red (630nm), green (560nm), amber(600nm) and blue (470nm) filters supplied.
• Uses standard 4 ml capacity square cuvettes.
• 5 cuvettes and lids supplied (optical polystyrene).
• LED light technology minimises drift.
• Single calibration / zero control.

Enzyme reaction (Protease) (Biology (11-14) ebook)
This is simple version of following an enzyme reaction. It could fit into work about digestion and digestive enzymes. Much more reliable than amylase and starch.

Beetroot membrane colorimeter (Biology (14-18) eBook)
Use a colorimeter to study the changes in a partially permeable membrane due to heat. Measure the intensity of the colour around the beetroot plugs. Could also be used to study membrane changes due to exposure to ethanol.

Protease digestion of Casein, simple enzyme study (Biology (14-18) eBook)
Us a colorimeter to follow the digestion of protein by a non specific protease over time. An introductory activity to get students used to the apparatus and method. Easier than amylase. Highly recommended.

Temperature and enzyme activity (protease) (Biology (14-18) eBook)
Milk powder suspension contains proteins that are poorly soluble giving the milk an opaque appearance. A non specific protease will digest the proteins and make the solution transparent. The progress of the reaction can be followed with a colorimeter. If the substrate is stored at different temperatures the effect of temperature on the reaction can be demonstrated.

pH and Enzyme activity (protease) (Biology (14-18) eBook)
Milk powder suspension is an opaque suspension of poorly soluble proteins. A non specific protease will digest the protein creating a transparent solution. A colorimeter can be used to follow the reaction. Adding drops of acid to the reaction allows study of the effect of pH on the rate, 

Enzyme activity and substrate concentration (protease) (Biology (14-18) eBook)
In this investigation the effect of increasing the substrate concentration on the speed of the reaction is considered. Uses a non specific protease to digest milk proteins and change opacity of the reaction mix.

Inhibition of enzyme activity by cobalt (Biology (14-18) eBook)
Many of the transition metals act as inhibitors of enzyme systems; in part this explains the toxicity of these metals. The experiment uses cobalt as an inhibitor as it is a readily available metal salt. Ideal extension activity for enzyme work, once mastered, try other metal salts.

Model of Michaelis-Menten (Biology (14-18) eBook)
A demonstration of how the Michaelis constant could be found for an enzyme system. Good extension or project practical.

Enzyme action (Amylase) (Biology (14-18) eBook)
Use a colorimeter to follow the classic amylase digestion of starch.

Enzymes and temperature (amylase) (Biology (14-18) eBook)
Change the temperature of the substrate to study the effect of amylase on starch digestion. Uses a colorimeter to follow the change in colour of the starch-iodine complex

Enzymes and pH (amylase) (Biology (14-18) eBook)
Use a colorimeter to follow the change of colour of the starch-iodine complex during the amylase digestion of starch. pH is the controlled variable.

Enzymes and substrate concentration (amylase) (Biology (14-18) eBook)
What is the effect of changing the substrate concentration on the amylase digestion of starch. Uses a colorimeter to follow the change in colour of the starch iodine complex as the digestion takes place

Estimation of glucose (colorimeter) (Biology (14-18) eBook)
An alternative method for measurement of glucose. Uses manganate solution and a colorimeter to record how long to decolourise. Application of Beer Lamberts law and creation of standards curves.

Glucose and Benedict's (Biology (14-18) eBook)
A more precise Benedict's test. Uses colorimeter to determine the depth of colour from the test. Application of Beer Lamberts law and use of calibration curves.

Why Does Fruit Brown (Biology (14-18) eBook)
look at why fruit browns using a colorimeter to create some numerical data for comparison

Thiosulphate rate of reaction investigation. (Chemistry (14-18) eBook)
This investigation is about rates of reaction, Uses the classic thiosulphate and acid reaction, but follows the rate with a data logging colorimeter

Ethanedioate autocatalysis (Chemistry (14-18) eBook)
Manganate(VII) ions in acid solution oxidise ethanedioate ions to carbon dioxide. As manganate(VII) ions are coloured deep purple, the reaction can be followed colorimetrically by monitoring the loss of the colour as the reaction progresses.

Beer's Law (Chemistry (14-18) eBook)
This experiment is effectively a demonstration of the principles of Beer’s law. The applications are very wide spread and include biomedical monitoring to quality assurance of beer.

Estimation of chlorine In water. (Chemistry (14-18) eBook)
Chlorine is added to pool water to prevent the spread of waterborne diseases. Chlorine levels need to measured to make the water safe from microorganisms and safe enough to swim in.

How much magnesium in a paper clip. (Chemistry (14-18) eBook)
Colorimetry is an analytical technique used to determine the concentrations of coloured substances in solution. It relies on the fact that a coloured substance absorbs light in direct proportion to concentration.

Stoichometry Of copper And EDTA. (Chemistry (14-18) eBook)
Stoichiometry is the study in chemistry of the ratio of the combining proportions of particles in a reaction. In this investigation copper is added to EDTA and the ratios of combination determined by colour density

Chemical equilibria: Calculationg Kc (Chemistry (14-18) eBook)
When Fe3+ ions and SCN- ions are combined they form the FeSCN2+ ion that is coloured blood red. The three ions form equilibrium with each other. The coloured product of the reaction can be measured indirectly by its absorbance in a Colorimeterand hence the Kc calculated

Estimating glucose in a solution (Chemistry (14-18) eBook)
Uses the time for a permanganate solution to discolour to create a concentration graph for estimation of glucose.